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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
03/10/2018 |
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Data da última atualização: |
03/10/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LIMA, A. M.; CUNHA, E. F. M.; ISHIDA, A. K. N.; PEREIRA, A. C. da C.; REIS, S. P. dos; SOUZA, C. R. B. de. |
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Afiliação: |
Aline Medeiros Lima, UFRA/UFPA; ELISA FERREIRA MOURA CUNHA, CPATU; ALESSANDRA KEIKO NAKASONE ISHIDA, CPATU; Angelo Cleiton da Costa Pereira, UFRA/UFPA; Sávio Pinho dos Reis, UFPA/UEPA; Cláudia Regina Batista de Souza, UFPA. |
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Título: |
Expression profiles of defense genes in cassava storage roots upon exposure to Phytopythium sp., causal agent of soft root rot disease. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Physiological and Molecular Plant Pathology, v. 104, p. 23-30, Dec. 2018. |
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DOI: |
10.1016/j.pmpp.2018.09.001 |
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Idioma: |
Inglês |
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Conteúdo: |
Cassava is one of the most important crops in Africa, Asia and Latin America, where millions of people depend on its starchy storage roots as their main source of energy. In Brazil, cassava is cultivated in all regions; however, soft root rot disease has hampered its production mainly in the Amazon region. The identification of Phytopythium sp., as the causal agent of soft root rot disease in cassava plants collected in Brazil, was recently reported. Here, our main aim was to evaluate changes in expression levels of genes possibly involved in compatible cassava- Phytopythium sp. interaction. Semi-quantitative RT-PCR assays were performed to evaluate the expression levels of genes coding for proteins with known functions in plant defense, such as enzymes involved in oxidative burst and the phenylpropanoid pathway, in detached cassava storage roots inoculated and noninoculated with pathogen. Our results showed that cassava responded to pathogen infection through up-regulation of genes coding for superoxide dismutase, phenylalanine ammonia-lyase, chalcone synthase, cysteine proteinase inhibitor and RING zinc finger protein. Furthermore, the expression pattern of the allergenic-related glutamic acid-rich protein and translationally controlled tumor protein genes in detached roots used here validated the absence of post-harvest physiological deterioration, a known endogenous disorder affecting the storage roots of cassava at 24?72 h after harvest. This is the first study of interaction between cassava and Phytopythium sp. at the molecular level contributing to understanding how cassava responds to pathogen infection, as well as to future strategies of molecular breeding regarding the tolerance to soft root rot disease. MenosCassava is one of the most important crops in Africa, Asia and Latin America, where millions of people depend on its starchy storage roots as their main source of energy. In Brazil, cassava is cultivated in all regions; however, soft root rot disease has hampered its production mainly in the Amazon region. The identification of Phytopythium sp., as the causal agent of soft root rot disease in cassava plants collected in Brazil, was recently reported. Here, our main aim was to evaluate changes in expression levels of genes possibly involved in compatible cassava- Phytopythium sp. interaction. Semi-quantitative RT-PCR assays were performed to evaluate the expression levels of genes coding for proteins with known functions in plant defense, such as enzymes involved in oxidative burst and the phenylpropanoid pathway, in detached cassava storage roots inoculated and noninoculated with pathogen. Our results showed that cassava responded to pathogen infection through up-regulation of genes coding for superoxide dismutase, phenylalanine ammonia-lyase, chalcone synthase, cysteine proteinase inhibitor and RING zinc finger protein. Furthermore, the expression pattern of the allergenic-related glutamic acid-rich protein and translationally controlled tumor protein genes in detached roots used here validated the absence of post-harvest physiological deterioration, a known endogenous disorder affecting the storage roots of cassava at 24?72 h after harvest. This is the first study of inter... Mostrar Tudo |
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Thesagro: |
Defesa Vegetal; Doença; Doença de Planta; Mandioca; Podridão Radicular. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02563naa a2200253 a 4500
001 2096813
005 2018-10-03
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.pmpp.2018.09.001$2DOI
100 1 $aLIMA, A. M.
245 $aExpression profiles of defense genes in cassava storage roots upon exposure to Phytopythium sp., causal agent of soft root rot disease.$h[electronic resource]
260 $c2018
520 $aCassava is one of the most important crops in Africa, Asia and Latin America, where millions of people depend on its starchy storage roots as their main source of energy. In Brazil, cassava is cultivated in all regions; however, soft root rot disease has hampered its production mainly in the Amazon region. The identification of Phytopythium sp., as the causal agent of soft root rot disease in cassava plants collected in Brazil, was recently reported. Here, our main aim was to evaluate changes in expression levels of genes possibly involved in compatible cassava- Phytopythium sp. interaction. Semi-quantitative RT-PCR assays were performed to evaluate the expression levels of genes coding for proteins with known functions in plant defense, such as enzymes involved in oxidative burst and the phenylpropanoid pathway, in detached cassava storage roots inoculated and noninoculated with pathogen. Our results showed that cassava responded to pathogen infection through up-regulation of genes coding for superoxide dismutase, phenylalanine ammonia-lyase, chalcone synthase, cysteine proteinase inhibitor and RING zinc finger protein. Furthermore, the expression pattern of the allergenic-related glutamic acid-rich protein and translationally controlled tumor protein genes in detached roots used here validated the absence of post-harvest physiological deterioration, a known endogenous disorder affecting the storage roots of cassava at 24?72 h after harvest. This is the first study of interaction between cassava and Phytopythium sp. at the molecular level contributing to understanding how cassava responds to pathogen infection, as well as to future strategies of molecular breeding regarding the tolerance to soft root rot disease.
650 $aDefesa Vegetal
650 $aDoença
650 $aDoença de Planta
650 $aMandioca
650 $aPodridão Radicular
700 1 $aCUNHA, E. F. M.
700 1 $aISHIDA, A. K. N.
700 1 $aPEREIRA, A. C. da C.
700 1 $aREIS, S. P. dos
700 1 $aSOUZA, C. R. B. de
773 $tPhysiological and Molecular Plant Pathology$gv. 104, p. 23-30, Dec. 2018.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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| Registros recuperados : 23 | |
| 6. |  | OLIVEIRA, H. S. de; LEMOS, O. F. de; SOUZA, C. R. B. de; MENEZES, I. C. de. Produção de plantas in vitro para adaptação de protocolos de micropropagação para diferentes cultivares de pimenteira-do-reino. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA UFRA, 2.; SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL (AVALIAÇÃO-2004), 8., 2005, Belém, PA. Ciência e tecnologia com inclusão social: anais. Belém, PA: UFRA: Embrapa Amazônia Oriental, 2005. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 8. | | OLIVEIRA, H. S. de; LEMOS, O. F. de; SOUZA, C. R. B. de; MENEZES, I. C. de. Micropropagação de espécies de pimenta nativas da Amazônia. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA UFRA, 2.; SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL (AVALIAÇÃO-2004), 8., 2005, Belém, PA. Ciência e tecnologia com inclusão social: anais. Belém, PA: UFRA: Embrapa Amazônia Oriental, 2005. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 12. | | OLIVEIRA, H. S. de; LEMOS, O. F. de; SOUZA, C. R. B. de; MENEZES, I. C. de; AMARAL, L. M. S. Produção in vitro de brotos enraizados de cultivares de pimenteira-do-reino (Piper nigrum). In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA UFRA, 4.; SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 10., 2007, Belém, PA. Anais... Belém, PA: UFRA: Embrapa Amazônia Oriental, 2007. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 15. | | COSTA, C. de N. M.; BRÍGIDA, A. B. S.; BORGES, B. do N.; MENEZES NETO, M. A. de; CARVALHO, L. J. C. B.; SOUZA, C. R. B. de. Levels of MeLEA3, a cDNA Sequence Coding for an Atypical Late Embryogenesis Abundant Protein in Cassava, Increase Under In Vitro Salt Stress Treatment. Plant Molecular Biology Reporter, v. 29, p. 997-1005, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | NASCIMENTO, S. B.; CASCARDO, J. C. de M.; MENEZES, I. C. de; DUARTE, M. de L. R.; DARNET, S.; HARADA, M. L.; SOUZA, C. R. B. de. Identifying sequences potentially related to resistance response of Piper tuberculatum to Fusarium solani f. sp. piperis by suppression subtractive hybridization. Protein & Peptide Letters, v. 16, n. 12, p. 1429-1434, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 17. | | NASCIMENTO, S. B.; MENEZES, I. C. de; COSTA, C. N. M.; MOREIRA, E. C. O.; DUARTE, M. L. R.; SOUZA, C. R. B. de. Isolamento e caracterização de genes potencialmente envolvidos na resistência a fusariose através de hibridização subtrativa de supressão. In: CONGRESSO BRASILEIRO DE GENÉTICA, 54., 2008, Salvador. Resumos... Salvador: SBG, 2008. p. 304.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 18. | | LIMA, A. M.; CUNHA, E. F. M.; ISHIDA, A. K. N.; PEREIRA, A. C. da C.; REIS, S. P. dos; SOUZA, C. R. B. de. Expression profiles of defense genes in cassava storage roots upon exposure to Phytopythium sp., causal agent of soft root rot disease. Physiological and Molecular Plant Pathology, v. 104, p. 23-30, Dec. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 19. | | OLIVEIRA, H. S. de; LEMOS, O. F. de; MENEZES, I. C. de; SOUZA, C. R. B. de; CARODSO, J. de N. O.; ALVES, S. A. O. Efeito do NAA e IBA no desenvolvimento e formação de mudas de cultivares de pimenteira-do-reino. In: REUNIÃO ANUAL DA SBPC, 59., 2007, Belém, PA. Resumos... Belém, PA: SBPC, [2007].| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 20. | | CARVALHO, L. J. C. B.; ALMEIDA, J. D. de; ANDERSON, J. V.; VIEIRA, E. A.; CHEN, S.; SOUZA, C. R. B. de; FUHRMANN, E.; SILVA, J. P. da. Studies on variation of carotenoid-proteins content in Cassava (Manihot esculenta Crantz) storage root reveal implications for breeding and the use of induced mutations. Plant Mutation Reports, v. 3, n. 1, p. 25-36, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 23 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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